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KMID : 0613820190290101086
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Journal of Life Science
2019 Volume.29 No. 10 p.1086 ~ p.1095
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Physiological Activities of Bioconversion Products Using Bacillus Subtillis KJ-3 and Their Mixtures
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Lee Jin-Young
Dong Jae-Kyung
Chung Yu-Seong
Kim Mi-Ryung
Kang Jae-Seon
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Abstract
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This research was performed to develop a new material consisting of a mixture of Red Allium cepa (RA) Cucurbita moschata duch (CM), and Angelica gigas Nakai (AG). RA and CM have low storage stability because of their high moisture content. Therefore, their major components were extracted and used for the research after a content analysis. In order to overcome these limitations, the quercetin from RA, β-carotene from CM, and decursin/decursinol angelate (D/DA) from AG were separately extracted, and the biochemical activity of each extract and mixture was compared. RA was bioconverted by the Bacillus subtillis KJ-3 (BS3) after ethanol extraction. After bioconversion, the quercetin content of RA was increased by 128.9%. β-carotene was detected in the CM ethanol extract and its content was very low concentrations at 0.2 mg/g. The AG ethanol extract (1 mg) contained 0.4146 mg and 0.3659 mg of D/DA, respectively. The purity of the D/DA was found to be about 78%. The flavonoid and polyphenol content of each extract and their mixtures (mixture 1 (RA:CM:AG = 5:2:3), mixture 2 (RA:CM: AG = 3:5:2), and mixture 3 (RA:CM:AG = 3:2:5)) were measured. In addition, the cell survival rate, anti-inflammatory activity, and antioxidant ability were also evaluated. In all the results, the antioxidant activity of mixture 3 was most effective. Therefore, these findings provide basic data for future food development using a 3:2:5 mixture of RA, CM, and AG.
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KEYWORD
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Angelica gigas nakai, Bacillus subtillis KJ-3, Bioconversion, Cucurbita moschata duch, Red allium cepa
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